This document specifies a method for the determination of the content of undissolved substances, referred to as total contamination, in neat fatty acid methyl esters (FAME). The working range is from 5 mg/kg to 27 mg/kg and it was established in an interlaboratory study by applying EN ISO 4259-1 [1]. This document in general is applicable to FAME having a kinematic viscosity not exceeding 8 mm2/s at 20 °C, or 5 mm2/s at 40 °C, e.g. as specified in EN 14214 [2]. This test method can be used for FAME having a kinematic viscosity exceeding 8 mm2/s at 20 °C, or 5 mm2/s at 40 °C, however in such cases the precision of the test method has not been determined. NOTE For the purposes of this document, the term “% (V/V)” is used to represent the volume fraction, φ, of a material. WARNING - The use of this document can involve hazardous materials, operations and equipment. This document does not purport to address all of the safety problems associated with its use. It is the responsibility of the user of this document to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.