This International Standard describes a method for determining the effects of substances on the reproduction of Folsomia candida by dermal and alimentary uptake in a defined artificial soil substrate. The method is not applicable to volatile substances, i.e. substances for which H (Henry's constant) or the air/water partition coefficient is greater than 1, or for which the vapour pressure exceeds 0,013 3 Pa at 25 °C. NOTE 1 The stability of the test substance cannot be assured over the test period. No allowance is made in the test method described for possible degradation of the test substance over the course of the experiment.. NOTE 2 Recommendations for adapting the method for comparing or monitoring soil quality are given in annex E.